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KMID : 0613820100200101427
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Journal of Life Science
2010 Volume.20 No. 10 p.1427 ~ p.1432
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Production of the Antiserum against Recombinant Envelop Protein, rVP466 for the Neutralization of White Spot Syndrome Virus (WSSV)
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Gong Soo-Jung
Kim Yeong-Jin
Choi Mi-Ran
Kim Sung-Koo
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Abstract
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This study was carried out to evaluate neutralization effects against WSSV using antiserum produced from recombinant envelop protein, rVP466 of WSSV. The VP466 gene of WSSV was cloned into pCold I expression vector and rVP466 was expressed in E. coli RIPL. The antiserum against rVP466 was produced in white rabbits (New Zealand white rabbit). The specific immunoreactivity to the antigen, rVP466, was confirmed by Western blot. The constant amounts of WSSV at 1×10⁴ diluted stocks were mixed with various antiserum concentrations and then injected to the muscle of shrimp, Penaeus chinensis, for the neutralization challenge. The shrimps challenged with WSSV as a positive control and those with the mixture of WSSV and preimmune serum as a preimmune control showed 100% cumulative mortality at 17 days post challenge and 83% at 25 days post challenge, respectively. The shrimps challenged with 3 different mixtures of WSSV and rVP466 antiserum at ratios of 1:0.01, 1:0.1 and 1:1 showed 73%, 53% and 46% cumulative mortalities at 25 days post challenge, respectively. These results indicated that WSSV could be neutralized by the rVP466 antiserum. These results suggest that envelop protein VP466 is involved in the initial step of WSSV infection in shrimp.
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KEYWORD
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White spot syndrome virus(WSSV), VP466, antiserum, neutralization
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